Batch and Continuous Extraction of Bromelain Enzyme from pineapple juice (Ananas comosus)
Reversed micelles, Bromelain, pineapple, Optimization, Response surface, COCO, Purification fold.
Bromelain is one of the vegetal proteases found in pineapple plant. It has numerous applications in food and pharmaceuticals. This study was focused on industrial large-scale bromelain purification technique (Reversed micelles) which will assist in determining the effect of processing conditions on the purification efficacy. The purification was carried out in batch extraction and a COCO simulation for continuous extraction. The cationic micellar solution was made of CTAB as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a best purification factor at the values of surfactant agent, co-solvent and KBr concentrations, pH of the back and forward extractions were, 152.18 mM, 10.53 % v/v, 1.24 M, 2.67 and 7.90, respectively. For the continuous operation, optimal process was determined using simulation. This optimal point led to a productivity of 96.128% and a purification fold of 10.87.
BINDHU.J. "Batch and Continuous Extraction of Bromelain Enzyme from pineapple juice (Ananas comosus)".INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT ISSN:2456-4184, Vol.7, Issue 11, pp.b600-b603, URL :https://ijnrd.org/papers/IJNRD2211170.pdf
Volume 7 Issue 11
Pages. b600-b603
